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Figure 3.
Summary of binding studies performed with various BET family proteins or their derived bromodomains with acetylated histone H3 and H4 peptides in vitro or with chromatin in live cells
Human (h) or mouse (m) bromodomains (BD1 and BD2) or BET (bromodomain and extraterminal domain) proteins purified from bacteria (bac) or present in nuclear extract (NE) or in living cells, such as 293, NIH3T3, HeLa or bone marrow-derived macrophages (BMMΦs), used for binding studies with acetylated histone H3 and H4 are indicated on the left. The assay used for individual analysis and the original reference (Ref.) reporting the results are listed on the right. Only positive interaction (+) is marked. Diacetylated residues in histone H4 required for cooperative binding are bracketed with stronger interaction indicated by thick lines. The two red and black dashed lines indicate the weakest interaction compared to other interactions depicted by bracketed solid lines. The crosstalk between H3S10 phosphorylation and H4K16ac is linked by a dashed blue arrow. ChIP, chromatin immunoprecipitation; IP, immunoprecipitation. |
