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Figure 2.
The predominantly monomeric precursor of HIV protease accompanied by lightly populated dimeric species
The blue lines represent some of the multitude of orientations in the transient encounter complexes that the second precursor subunit may adopt relative to the monomer plotted. The lines connect the Cα atoms of Gly27 of the active site and Ile62 on the distant side of the protomer. In the mature dimer, this vector runs horizontally in the middle on the right. The red coloring marks segments for which the proton NMR peaks are broadened by intermolecular paramagnetic relaxation enhancements from nitroxide labeling of half of the molecules in the samples of the truncated precursor. Termini are labeled. |
