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Specific biomarkers for stochastic division patterns and starvation-induced quiescence under limited glucose levels in fission yeast.
FEBS J. 2011 Apr; 278(8):1299-315
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20 Apr 2011
Confirmation, New Finding
DOI: 10.3410/f.9805958.10506056
This very detailed study of the growth and metabolism of fission yeast cells in response to glucose limitation, using microfluidics and quantitative metabolomics, provides a valuable basis to future analyses of glucose sensing and cellular ageing.
Fission yeast shows surprising resilience in growth to...Continue reading
Fission yeast shows surprising resilience in growth to...Continue reading
This very detailed study of the growth and metabolism of fission yeast cells in response to glucose limitation, using microfluidics and quantitative metabolomics, provides a valuable basis to future analyses of glucose sensing and cellular ageing.
Fission yeast shows surprising resilience in growth to a wide range of glucose concentrations, down to the low levels found in human blood. At concentrations below 4.4mM, cell division becomes highly variable and shows intriguing signs of epigenetic inheritance across generations. Careful analyses of the rapid dynamic changes of specific metabolites give insight into cellular physiology and oxidative stress protection as a function of glucose levels.
Fission yeast shows surprising resilience in growth to a wide range of glucose concentrations, down to the low levels found in human blood. At concentrations below 4.4mM, cell division becomes highly variable and shows intriguing signs of epigenetic inheritance across generations. Careful analyses of the rapid dynamic changes of specific metabolites give insight into cellular physiology and oxidative stress protection as a function of glucose levels.
Disclosures
None declared
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Bahler J: F1000Prime Recommendation of [Pluskal T et al., FEBS J 2011, 278(8):1299-315]. In F1000Prime, 20 Apr 2011; DOI: 10.3410/f.9805958.10506056. F1000Prime.com/9805958#eval10506056
F1000Prime Recommendations, Dissents and Comments for [Pluskal T et al., FEBS J 2011, 278(8):1299-315]. In F1000Prime, 22 May 2013; F1000Prime.com/9805958
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Glucose as a source of energy is centrally important to our understanding of life. We investigated the cell division-quiescence behavior of the fission yeast Schizosaccharomyces pombe under a wide range of glucose concentrations (0-111 mM). The mode of S. pombe cell division under a microfluidic perfusion system was surprisingly normal under highly diluted glucose concentrations (5.6 mM, 1/20 of the standard medium, within human blood sugar levels). Division became stochastic, accompanied by a curious division-timing inheritance, in 2.2-4.4 mM glucose. A critical transition from division to quiescence occurred within a narrow range of concentrations (2.2-1.7 mM). Under starvation (1.1 mM) conditions, cells were mostly quiescent and only a small population of cells divided. Under fasting (0 mM) conditions, division was immediately arrested with a short chronological lifespan (16 h). When cells were first glucose starved prior to fasting, they possessed a substantially extended lifespan (∼14 days). We employed a quantitative metabolomic approach for S. pombe cell extracts, and identified specific metabolites (e.g. biotin, trehalose, ergothioneine, S-adenosyl methionine and CDP-choline), which increased or decreased at different glucose concentrations, whereas nucleotide triphosphates, such as ATP, maintained high concentrations even under starvation. Under starvation, the level of S-adenosyl methionine increased sharply, accompanied by an increase in methylated amino acids and nucleotides. Under fasting, cells rapidly lost antioxidant and energy compounds, such as glutathione and ATP, but, in fasting cells after starvation, these and other metabolites ensuring longevity remained abundant. Glucose-starved cells became resistant to 40 mM H(2)O(2) as a result of the accumulation of antioxidant compounds.
© 2011 The Authors Journal compilation © 2011 FEBS.
DOI: 10.1111/j.1742-4658.2011.08050.x
PMID: 21306563
