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Remodelling of the Vibrio cholerae membrane by incorporation of exogenous fatty acids from host and aquatic environments.
Mol Microbiol. 2011 Feb; 79(3):716-28
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07 Mar 2011
DOI: 10.3410/f.8468957.9290055
This study elegantly shows that Vibrio species possess the evidently unique ability to acquire fatty acids from different environments and incorporate them into membrane phospholipids.
The authors find that exposure of V. cholerae to physiologically relevant concentrations of bile results in significant...Continue reading
The authors find that exposure of V. cholerae to physiologically relevant concentrations of bile results in significant...Continue reading
This study elegantly shows that Vibrio species possess the evidently unique ability to acquire fatty acids from different environments and incorporate them into membrane phospholipids.
The authors find that exposure of V. cholerae to physiologically relevant concentrations of bile results in significant alteration of its phospholipid profile. Similar alterations were also observed for other Vibrio species in the presence of bile, but were not observed for Escherichia coli and Salmonella. The authors also show that the major components of bile are not the cause of the observed changes, but that V. cholerae assimilates long-chain polyunsaturated fatty acids, minor components of bile. Bile exposure also causes V. cholerae to produce large quantities of a new phospholipid, identified to be lyso-phosphatidylethanolamine, constituting from 25-30% of the total lipids. The authors also demonstrate that V. cholerae could acquire fatty acids from marine sediment, indicating that this estuarine organism may adopt specific phospholipid profiles as a mechanism of adapting to the various environments it inhabits.
The authors find that exposure of V. cholerae to physiologically relevant concentrations of bile results in significant alteration of its phospholipid profile. Similar alterations were also observed for other Vibrio species in the presence of bile, but were not observed for Escherichia coli and Salmonella. The authors also show that the major components of bile are not the cause of the observed changes, but that V. cholerae assimilates long-chain polyunsaturated fatty acids, minor components of bile. Bile exposure also causes V. cholerae to produce large quantities of a new phospholipid, identified to be lyso-phosphatidylethanolamine, constituting from 25-30% of the total lipids. The authors also demonstrate that V. cholerae could acquire fatty acids from marine sediment, indicating that this estuarine organism may adopt specific phospholipid profiles as a mechanism of adapting to the various environments it inhabits.
Disclosures
None declared
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DiRita V and Matson J: F1000Prime Recommendation of [Giles DK et al., Mol Microbiol 2011, 79(3):716-28]. In F1000Prime, 07 Mar 2011; DOI: 10.3410/f.8468957.9290055. F1000Prime.com/8468957#eval9290055
F1000Prime Recommendations, Dissents and Comments for [Giles DK et al., Mol Microbiol 2011, 79(3):716-28]. In F1000Prime, 25 May 2013; F1000Prime.com/8468957
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The Gram-negative bacteria Vibrio cholerae poses significant public health concerns by causing an acute intestinal infection afflicting millions of people each year. V. cholerae motility, as well as virulence factor expression and outer membrane protein production, has been shown to be affected by bile. The current study examines the effects of bile on V. cholerae phospholipids. Bile exposure caused significant alterations to the phospholipid profile of V. cholerae but not of other enteric pathogens. These changes consisted of a quantitative increase and migratory difference in cardiolipin, decreases in phosphatidylglycerol and phosphatidylethanolamine, and the dramatic appearance of an unknown phospholipid determined to be lyso-phosphatidylethanolamine. Major components of bile were not responsible for the observed changes, but long-chain polyunsaturated fatty acids, which are minor components of bile, were shown to be incorporated into phospholipids of V. cholerae. Although the bile-induced phospholipid profile was independent of the V. cholerae virulence cascade, we identified another relevant environment in which V. cholerae assimilates unique fatty acids into its membrane phospholipids - marine sediment. Our results suggest that Vibrio species possess unique machinery conferring the ability to take up a wider range of exogenous fatty acids than other enteric bacteria.
© 2010 Blackwell Publishing Ltd.
DOI: 10.1111/j.1365-2958.2010.07476.x
PMID: 21255114
