RNA SHAPE analysis in living cells.
Nat Chem Biol. 2012 Nov 25
David Evans
15 Jan 2013
|
New Finding, Technical Advance
In this manuscript, Spitale and colleagues develop and convincingly demonstrate that 2-methylnicotinic acid imidazolide (NAI) can be used to acylate the 2'-hydroxyl of RNA in a range of living cells--mammalian, fly, yeast and bacterial--thereby enabling 2'-hydroxyl acylation analyzed by primer extension (SHAPE) mapping of RNA structures...
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