RNA SHAPE analysis in living cells.
Nat Chem Biol. 2012 Nov 25
Spitale RC, Crisalli P, Flynn RA, Torre EA, Kool ET, Chang HY. Nat Chem Biol. 2012 Nov 25
In this manuscript, Spitale and colleagues develop and convincingly demonstrate that 2-methylnicotinic acid imidazolide (NAI) can be used to acylate the 2'-hydroxyl of RNA in a range of living cells--mammalian, fly, yeast and bacterial--thereby enabling 2'-hydroxyl acylation analyzed by primer extension (SHAPE) mapping of RNA structures...
Get instant access to Article Recommendations from more than 5,000 expert scientists and clinical researchers, assisted by 5,000 associates, by taking out a monthly subscription. The first month of your subscription will be free of charge.
Start your personal subscription today.Subscribe for $9.95/month
Send a recommendation to your institution's librarian or information manager to request an extended free trial for all users.Recommend to your Librarian
If you are a librarian or information manager, you may request an extended free trial for your academic or corporate institution.Request an Institutional Trial
your institution might be eligible for free access via our sponsorship scheme. Check our sponsorship page.
If you think you should be able to access this content, please contact us.