Fast production of homogeneous recombinant RNA--towards large-scale production of RNA.
Nucleic Acids Res. 2012 Mar 28
Nelissen FH, Leunissen EH, van de Laar L, Tessari M, Heus HA, Wijmenga SS. Nucleic Acids Res. 2012 Mar 28
The preparation of large quantities of RNA on the scale of tens of milligrams is required for systematic studies of the structure and function of RNA molecules. Compared with laborious in vitro T7 transcription or costly chemical synthesis methods, this manuscript describes a generally applicable and improved recombinant RNA technology...
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