Live cell multicolor imaging of lipid droplets with a new dye, LD540.
Traffic. 2009 Nov; 10(11):1579-84
This article PDF usually requires a subscription to access but you can receive the PDF, at no cost, through a special arrangement between Springer Science+Business Media and Faculty of 1000 for our Faculty Members who act as reviewers. This PDF is provided to you only for the purposes of reviewing the article while you consider preparing a recommendation of the article for F1000Prime. Other than for this purpose you warrant that you will treat the PDF as confidential. Furthermore you warrant that you will not use the PDF for any other purpose, especially but not limited to forwarding, copying, selling, distributing it whether commercially or non-commercially and that you will not make it available on any website for any other purpose.
In this exciting report, the efficacy of a new Bodipy 493/503-derived lipophilic dye (LD540) for the study of lipid droplet (LD) biology is described.
The obesity epidemic in the Western world, when considered along with its associated health problems, has generated intense interest in the metabolism of LDs. Once considered a mere phase separation of neutral lipids and sterol esters from the cytosolic milieu, LDs are now considered to be bona fide organelles subject to complex metabolic control. This study shows that LD540 is a superior LD vital dye on the basis of its impressive specificity and photostability relative to currently used dyes such as Nile Red or Bodipy 493/503. The unique spectral properties of LD540 also make multicolor imaging possible. In summary, this report describes a valuable new tool for visualizing LDs, and LD dynamics, in living cells.
Bankaitis V and Ren J: F1000Prime Recommendation of [Spandl J et al., Traffic 2009, 10(11):1579-84]. In F1000Prime, 30 Nov 2010; DOI: 10.3410/f.6471961.6593060. F1000Prime.com/6471961#eval6593060
F1000Prime Recommendations, Dissents and Comments for [Spandl J et al., Traffic 2009, 10(11):1579-84]. In F1000Prime, 24 May 2015; F1000Prime.com/6471961
Get the most out of F1000Prime - attend a live online demonstration.
Please choose one of the following time zones:
Want to become an
has been added to your "Faculty I'm Following" page in MyF1000
Follow/Unfollow any Faculty via their recommendations, biography pages, or MyF1000
If you've forgotten your password, please enter your email address below and we'll send you instructions on how to reset your password.
The email address should be the one you originally registered with F1000.
You registered with F1000 via Google, so we cannot reset your password.
To sign in, please click here.
If you still need help with your Google account password, please click here.
You registered with F1000 via Facebook, so we cannot reset your password.
To sign in, please click here.
If you still need help with your Facebook account password, please click here.
We have sent an email to , please follow the instructions to reset your password.
If you don't receive this email, please check your spam filters and/or contact firstname.lastname@example.org.