Live cell multicolor imaging of lipid droplets with a new dye, LD540.
Traffic. 2009 Nov; 10(11):1579-84
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In this exciting report, the efficacy of a new Bodipy 493/503-derived lipophilic dye (LD540) for the study of lipid droplet (LD) biology is described.
The obesity epidemic in the Western world, when considered along with its associated health problems, has generated intense interest in the metabolism of LDs. Once considered a mere phase separation of neutral lipids and sterol esters from the cytosolic milieu, LDs are now considered to be bona fide organelles subject to complex metabolic control. This study shows that LD540 is a superior LD vital dye on the basis of its impressive specificity and photostability relative to currently used dyes such as Nile Red or Bodipy 493/503. The unique spectral properties of LD540 also make multicolor imaging possible. In summary, this report describes a valuable new tool for visualizing LDs, and LD dynamics, in living cells.
Bankaitis V and Ren J: F1000Prime Recommendation of [Spandl J et al., Traffic 2009, 10(11):1579-84]. In F1000Prime, 30 Nov 2010; DOI: 10.3410/f.6471961.6593060. F1000Prime.com/6471961#eval6593060
F1000Prime Recommendations, Dissents and Comments for [Spandl J et al., Traffic 2009, 10(11):1579-84]. In F1000Prime, 17 Sep 2014; F1000Prime.com/6471961
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