Deciphering protein kinase specificity through large-scale analysis of yeast phosphorylation site motifs.
Sci Signal. 2010; 3(109):ra12
Mok J, Kim PM, Lam HY, Piccirillo S, ..., Andrews BJ, Gerstein MB, Snyder M, Turk BE. Mok J, Kim PM, Lam HY, Piccirillo S, Zhou X, Jeschke GR, Sheridan DL, Parker SA, Desai V, Jwa M, Cameroni E, Niu H, Good M, Remenyi A, Ma JL, Sheu YJ, Sassi HE, Sopko R, Chan CS, De Virgilio C, Hollingsworth NM, Lim WA, Stern DF, Stillman B, Andrews BJ, Gerstein MB, Snyder M, Turk BE. Sci Signal. 2010; 3(109):ra12
This paper presents an impressive volume of data defining in vitro phosphorylation site motifs for yeast kinases through a clever peptide-based screening strategy. Integrating this approach with computational methods, the authors identify target-site motifs for approximately half the known kinases in yeast, providing an excellent...
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