Tracking the dynamic interplay between bacterial and host factors during pathogen-induced vacuole rupture in real time.
Cell Microbiol. 2010 Apr 1; 12(4):545-56
This article PDF usually requires a subscription to access but you can receive the PDF, at no cost, through a special arrangement between Springer Science+Business Media and Faculty of 1000 for our Faculty Members who act as reviewers. This PDF is provided to you only for the purposes of reviewing the article while you consider preparing a recommendation of the article for F1000Prime. Other than for this purpose you warrant that you will treat the PDF as confidential. Furthermore you warrant that you will not use the PDF for any other purpose, especially but not limited to forwarding, copying, selling, distributing it whether commercially or non-commercially and that you will not make it available on any website for any other purpose.
I found this article interesting because the authors developed a novel fluorescence microscopy methodology to detect rupture of the phagosome membrane by intracellular bacterial pathogens at the single cell level.
By combining the use of a chimeric protein AIDA-beta-lactamase, FRET, microscopy and an automated algorithm the authors were able to detect bacterial escape from phagosomes into the cytoplasm in real time. This novel procedure is temporally sensitive compared to other methods used to detect bacterial escape, like comet tail formation. Additionally, the use of fluorescent fusions to different host cell proteins helped them to identify multiple proteins recruited during the invasion and vacuole escape processes (i.e. in Shigella escape, RhoA, Rac1 and galectin-3 are recruited). Using this system, the authors were also able to show the role of Salmonella effectors PipB2 and SifA in vacuole stabilization. This method is limited so far to Gram-negative bacteria but could potentially be adapted for other pathogenic bacteria.
Steele-Mortimer O and Ibarra J: F1000Prime Recommendation of [Ray K et al., Cell Microbiol 2010, 12(4):545-56]. In F1000Prime, 13 Apr 2010; DOI: 10.3410/f.2886956.2555054. F1000Prime.com/2886956#eval2555054
F1000Prime Recommendations, Dissents and Comments for [Ray K et al., Cell Microbiol 2010, 12(4):545-56]. In F1000Prime, 31 Jul 2014; F1000Prime.com/2886956
Get the most out of F1000Prime - attend a live online demonstration.
Please choose one of the following time zones:
Want to become an
has been added to your "Faculty I'm Following" page in MyF1000
Follow/Unfollow any Faculty via their recommendations, biography pages, or MyF1000
If you've forgotten your password, please enter your email address below and we'll send you instructions on how to reset your password.
The email address should be the one you originally registered with F1000.
You registered with F1000 via Google, so we cannot reset your password.
To sign in, please click here.
If you still need help with your Google account password, please click here.
You registered with F1000 via Facebook, so we cannot reset your password.
To sign in, please click here.
If you still need help with your Facebook account password, please click here.
We have sent an email to , please follow the instructions to reset your password.
If you don't receive this email, please check your spam filters and/or contact email@example.com.