Cold-induced silencing by long antisense transcripts of an Arabidopsis Polycomb target.
Nature. 2009 Dec 10; 462(7274):799-802
This article PDF usually requires a subscription to access but you can receive the PDF, at no cost, through a special arrangement between Springer Science+Business Media and Faculty of 1000 for our Faculty Members who act as reviewers. This PDF is provided to you only for the purposes of reviewing the article while you consider preparing a recommendation of the article for F1000Prime. Other than for this purpose you warrant that you will treat the PDF as confidential. Furthermore you warrant that you will not use the PDF for any other purpose, especially but not limited to forwarding, copying, selling, distributing it whether commercially or non-commercially and that you will not make it available on any website for any other purpose.
This paper reports an unexpected finding that cold-treated plants induce long, non-coding antisense transcripts of the Arabidopsis floral repressor FLC, which silence the expression of their sense counterparts.
FLC is the central floral repressor that transcriptionally down-regulates several genes required for flowering. It is well known that exposure of plants to prolonged cold (i.e. vernalization) attenuates this FLC-dependent floral repression through the Polycomb-mediated epigenetic silencing of the FLC locus. The authors discovered that, in addition to this well-characterized mechanism, cold treatment induces production of long antisense transcripts designated COOLAIR that overlap the entire FLC coding region. The cold-induced upregulation of COOLAIR coincides with reduction of FLC mRNA, suggesting that such non-coding antisense transcripts play an indispensable role in the FLC transcriptional silencing. More importantly, it was shown that incorporation of the putative COOLAIR promoter sequence, which corresponds to the 3' region of FLC, downstream of the green fluorescent protein (GFP) reporter gene is sufficient to repress the reporter expression in a cold-responsive manner. To our knowledge, this is the first description of long antisense transcripts involved in transcriptional silencing in plants. In the future, it would be interesting to see what fraction of the plant genome is subject to regulation by such long antisense transcript-mediated gene silencing.
Citovsky V and Magori S: F1000Prime Recommendation of [Swiezewski S et al., Nature 2009, 462(7274):799-802]. In F1000Prime, 18 Jan 2010; DOI: 10.3410/f.1491956.974055. F1000Prime.com/1491956#eval974055
F1000Prime Recommendations, Dissents and Comments for [Swiezewski S et al., Nature 2009, 462(7274):799-802]. In F1000Prime, 25 Oct 2014; F1000Prime.com/1491956
Get the most out of F1000Prime - attend a live online demonstration.
Please choose one of the following time zones:
Want to become an
has been added to your "Faculty I'm Following" page in MyF1000
Follow/Unfollow any Faculty via their recommendations, biography pages, or MyF1000
If you've forgotten your password, please enter your email address below and we'll send you instructions on how to reset your password.
The email address should be the one you originally registered with F1000.
You registered with F1000 via Google, so we cannot reset your password.
To sign in, please click here.
If you still need help with your Google account password, please click here.
You registered with F1000 via Facebook, so we cannot reset your password.
To sign in, please click here.
If you still need help with your Facebook account password, please click here.
We have sent an email to , please follow the instructions to reset your password.
If you don't receive this email, please check your spam filters and/or contact firstname.lastname@example.org.