Trafficking of MHC class II in dendritic cells is dependent on but not regulated by degradation of its associated invariant chain.
Traffic. 2010 Mar; 11(3):324-31
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The authors warn that certain culture conditions can artificially increase the pH and alter protease activity of the endocytic route. This may be the cause of controversial findings pertaining to the regulation of endosomal pH and antigen presentation by dendritic cells (DCs).
Medium supplemented with glutamine (Gln) accumulates NH4Cl, which artificially raises the endosomal pH of cells in culture. The authors illustrate the consequences of this effect by analyzing the kinetics of MHC II-associated invariant chain (Ii) degradation (a process influenced by endosomal pH) and MHC II ubiquitination (which requires Ii proteolysis) in DCs. The results demonstrate that Gln decomposition alters these two processes. This study contributes to solve some long-standing controversies in the fields of antigen presentation and DC biology by demonstrating that reported changes in Ii degradation and endosomal pH during DC activation may be due to Gln decomposition. The importance of this paper extends to other studies of biological phenomena in which the regulation of endosomal pH and protease activity is relevant. The authors provide recommendations to prevent the formation of Gln decomposition products.
Villadangos J: F1000Prime Recommendation of [ten Broeke T et al., Traffic 2010, 11(3):324-31]. In F1000Prime, 18 Jan 2010; DOI: 10.3410/f.1489956.971054. F1000Prime.com/1489956#eval971054
F1000Prime Recommendations, Dissents and Comments for [ten Broeke T et al., Traffic 2010, 11(3):324-31]. In F1000Prime, 24 Apr 2014; F1000Prime.com/1489956
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