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The Translocation Selectivity of the Kinesins that Mediate Neuronal Organelle Transport.
Traffic. 2011 Dec 28
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23 Feb 2012
Greg Smith
F1000 Cell Biology
Northwestern University Feinberg School of Medicine,
Chicago, IL, USA.
F1000 Cell Biology
Northwestern University Feinberg School of Medicine,
Chicago, IL, USA.
Sofia Zaichick
F1000 Cell Biology
Northwestern University Feinberg School of Medicine,
Chicago, IL, USA.
F1000 Cell Biology
Northwestern University Feinberg School of Medicine,
Chicago, IL, USA.
Confirmation, New Finding
DOI: 10.3410/f.13460956.15377069
This study examines the preferential targeting of fourteen long-range transport kinesins in cultured hippocampal neurons. The findings support the hypothesis that kinesin motors have intrinsic targeting properties that may contribute to polarized transport of cargoes to axons, but are also consistent...Continue reading
This study examines the preferential targeting of fourteen long-range transport kinesins in cultured hippocampal neurons. The findings support the hypothesis that kinesin motors have intrinsic targeting properties that may contribute to polarized transport of cargoes to axons, but are also consistent with additional targeting mechanisms contributing to polarity, particularly regarding dendritic localization.
The authors used constitutively active, fluorescently tagged kinesins transiently expressed in hippocampal neurons. Of the fourteen kinesins studied, only seven selectively translocated into axons. The study also addressed dynamics of preferential kinesin distribution at different stages during neuron development. For a subset of kinesin, axon targeting selectivity was dependent upon the stage of neural development. Taxol treatment, which is known to enhance posttranslational modification of tubulin and to allow for accumulation of KIF5C at neurite tips, affected only a subset of kinesins. This supports that some kinesins recognize tubulin modifications, which, in concert with other factors, contributes to cargo transport selectivity.
The authors used constitutively active, fluorescently tagged kinesins transiently expressed in hippocampal neurons. Of the fourteen kinesins studied, only seven selectively translocated into axons. The study also addressed dynamics of preferential kinesin distribution at different stages during neuron development. For a subset of kinesin, axon targeting selectivity was dependent upon the stage of neural development. Taxol treatment, which is known to enhance posttranslational modification of tubulin and to allow for accumulation of KIF5C at neurite tips, affected only a subset of kinesins. This supports that some kinesins recognize tubulin modifications, which, in concert with other factors, contributes to cargo transport selectivity.
Disclosures
None declared
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Smith G and Zaichick S: F1000Prime Recommendation of [Huang CF and Banker G, Traffic 2011]. In F1000Prime, 23 Feb 2012; DOI: 10.3410/f.13460956.15377069. F1000Prime.com/13460956#eval15377069
F1000Prime Recommendations, Dissents and Comments for [Huang CF and Banker G, Traffic 2011]. In F1000Prime, 20 May 2013; F1000Prime.com/13460956
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Polarized kinesin-driven transport is crucial for development and maintenance of neuronal polarity. Kinesins are thought to recognize biochemical differences between axonal and dendritic microtubules in order to deliver their cargoes to the appropriate domain. To identify kinesins that mediate polarized transport, we prepared constitutively active versions of all the kinesins implicated in vesicle transport and expressed them in cultured hippocampal neurons. Seven kinesins translocated preferentially to axons and five translocated into both axons and dendrites. None translocated selectively to dendrites. Highly homologous members of the same subfamily displayed distinctly different translocation preferences and were differentially regulated during development. By expressing chimeric kinesins, we identified two microtubule-binding elements within the motor domain that are important for selective translocation. We also discovered elements in the dimerization domain of Kinesin-2 motors that contribute to their selective translocation. These observations indicate that selective interactions between kinesin motor domains and microtubules can account for polarized transport to the axon, but not for selective dendritic transport.
© 2011 John Wiley & Sons A/S.
DOI: 10.1111/j.1600-0854.2011.01325.x
PMID: 22212743
