Tracking single hematopoietic stem cells in vivo using high-throughput sequencing in conjunction with viral genetic barcoding.
Nat Biotechnol. 2011 Oct; 29(10):928-33
In this technological tour-de-force, the authors utilize new technology to address the differentiation of hematopoietic stem cells following transplantation in a mouse model.
The results confirm previous studies conducted with single-cell transfers, but with a far more robust data set and much higher density. In addition, the new approach demonstrates a marked bias for stem cell contributions to either B lymphocyte/granulocyte lineages or to B lymphocyte/T lymphocyte lineages. Further, the analysis shows that individual stem cells do not contribute equally to all blood lineages in irradiated recipient mice.
The results of this study, therefore, both confirm previous studies as well as establish new insights into the hematopoietic hierarchy.
Spangrude G: F1000Prime Recommendation of [Lu R et al., Nat Biotechnol 2011, 29(10):928-33]. In F1000Prime, 07 Oct 2011; DOI: 10.3410/f.13336001.14702117. F1000Prime.com/13336001#eval14702117
F1000Prime Recommendations, Dissents and Comments for [Lu R et al., Nat Biotechnol 2011, 29(10):928-33]. In F1000Prime, 10 Dec 2013; F1000Prime.com/13336001
Nat Med. 2002 Jun; 8(6):607-12
Cell Stem Cell. 2007 Aug 16; 1(2):218-29
Immunity. 2003 May; 18(5):699-711
Nat Biotechnol. 2005 Jan; 23(1):69-74
Nat Immunol. 2011 Feb; 12(2):129-36
Science. 2009 Nov 6; 326(5954):818-23
Blood. 2009 Jan 15; 113(3):546-54
Cell Stem Cell. 2008 Mar 6; 2(3):252-63
Cell. 2004 Jul 23; 118(2):149-61
Disentangling cellular heterogeneity is a challenge in many fields, particularly in the stem cell and cancer biology fields. Here we demonstrate how to combine viral genetic barcoding with high-throughput sequencing to track single cells in a heterogeneous population. We use this technique to track the in vivo differentiation of unitary hematopoietic stem cells (HSCs). The results are consistent with single-cell transplantation studies but require two orders of magnitude fewer mice. In addition to its high throughput, the high sensitivity of the technique allows for a direct examination of the clonality of sparse cell populations such as HSCs. We show how these capabilities offer a clonal perspective of the HSC differentiation process. In particular, our data suggest that HSCs do not equally contribute to blood cells after irradiation-mediated transplantation, and that two distinct HSC differentiation patterns co-exist in the same recipient mouse after irradiation. This technique can be applied to any virus-accessible cell type for both in vitro and in vivo processes.
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