Tracking single hematopoietic stem cells in vivo using high-throughput sequencing in conjunction with viral genetic barcoding.
Nat Biotechnol. 2011 Oct; 29(10):928-33
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In this technological tour-de-force, the authors utilize new technology to address the differentiation of hematopoietic stem cells following transplantation in a mouse model.
The results confirm previous studies conducted with single-cell transfers, but with a far more robust data set and much higher density. In addition, the new approach demonstrates a marked bias for stem cell contributions to either B lymphocyte/granulocyte lineages or to B lymphocyte/T lymphocyte lineages. Further, the analysis shows that individual stem cells do not contribute equally to all blood lineages in irradiated recipient mice.
The results of this study, therefore, both confirm previous studies as well as establish new insights into the hematopoietic hierarchy.
Spangrude G: F1000Prime Recommendation of [Lu R et al., Nat Biotechnol 2011, 29(10):928-33]. In F1000Prime, 07 Oct 2011; DOI: 10.3410/f.13336001.14702117. F1000Prime.com/13336001#eval14702117
F1000Prime Recommendations, Dissents and Comments for [Lu R et al., Nat Biotechnol 2011, 29(10):928-33]. In F1000Prime, 22 Sep 2014; F1000Prime.com/13336001
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