Grab a Golgi: laser trapping of Golgi bodies reveals in vivo interactions with the endoplasmic reticulum.
Traffic. 2009 May; 10(5):567-71
I found this paper very interesting as its results strongly imply that Golgi stacks are attached to the tubules of ER (endoplasmic reticulum) in plant cells. These findings help explain earlier findings that the domains of the ER that are involved in protein export are associated with motile Golgi stacks.
How can motile Golgi stacks receive cargo from the ER while they are moving? A characteristic of plant Golgi stacks is that they are motile. It has been shown that fluorescent protein fusions to COPII (coat protein complex II) proteins label structures that moved with the Golgi and that the Golgi could receive cargo from the ER during stationary and non-stationary phases. These findings raise the question, how is delivery of COPII carriers achieved if the Golgi moves over the ER surface? Using laser optical tweezers, Sparkes et al. have shown that Golgi stacks are associated with the ER and that the ER tubules can be remodeled to follow a Golgi trapped and dragged in a cell with the tweezers. These findings strongly imply that Golgi stacks may move with the ER rather than over it and are consistent with a model in which COPII carriers are generated at an ER/Golgi interface that is relatively static.
Brandizzi F: F1000Prime Recommendation of [Sparkes IA et al., Traffic 2009, 10(5):567-71]. In F1000Prime, 17 Dec 2009; DOI: 10.3410/f.1282050.749147. F1000Prime.com/1282050#eval749147
F1000Prime Recommendations, Dissents and Comments for [Sparkes IA et al., Traffic 2009, 10(5):567-71]. In F1000Prime, 10 Dec 2013; F1000Prime.com/1282050
Nat Genet. 2006 Oct; 38(10):1192-7
Stephens DJ. EMBO Rep. 2003 Feb; 4(2):210-7
Plant Cell. 2013 Sep; 25(9):3434-49
In many vacuolate plant cells, individual Golgi bodies appear to be attached to tubules of the pleiomorphic cortical endoplasmic reticulum (ER) network. Such observations culminated in the controversial mobile secretory unit hypothesis to explain transport of cargo from the ER to Golgi via Golgi attached export sites. This proposes that individual Golgi bodies and an attached-ER exit machinery move over or with the surface of the ER whilst collecting cargo for secretion. By the application of infrared laser optical traps to individual Golgi bodies within living leaf cells, we show that individual Golgi bodies can be micromanipulated to reveal their association with the ER. Golgi bodies are physically attached to ER tubules and lateral displacement of individual Golgi bodies results in the rapid growth of the attached ER tubule. Remarkably, the ER network can be remodelled in living cells simply by movement of laser trapped Golgi dragging new ER tubules through the cytoplasm and new ER anchor sites can be established. Finally, we show that trapped Golgi ripped off the ER are 'sticky' and can be docked on to and attached to ER tubules, which will again show rapid growth whilst pulled by moving Golgi.
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