Real-time imaging of notch activation with a luciferase complementation-based reporter.
Sci Signal. 2011; 4(181):rs7
Pamela Stanley
25 Jul 2011
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Technical Advance
This paper describes an elegant and highly sensitive Notch signaling assay that may be used to follow Notch ligand-dependent and ligand-independent signaling in live cells in real time, and to identify Notch pathway modulators in high throughput screens.
The basis of the assay is the use of split luciferase (Luc) fragments (N- and C-Luc)...
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