Restriction enzyme-free mutagenesis via the light regulation of DNA polymerization.
Nucleic Acids Res. 2009 May; 37(8):e58
Scott K Silverman
14 May 2009
|
Technical Advance
Alternative mutagenesis methods are always welcome. Here the authors describe how a photocaged (light-sensitive) T nucleotide enables plasmid mutagenesis without using any restriction enzymes.
In the caged T that the authors previously developed {1,2}, the caging group blocks the Watson-Crick face of the nucleobase and inhibits hybridization....
INDIVIDUAL SUBSCRIPTIONS
Get instant access to Article Recommendations from more than 5,000 expert scientists and clinical researchers, assisted by 5,000 associates, by taking out a monthly subscription. The first month of your subscription will be free of charge.
Start your personal subscription today.
Subscribe for $9.95/monthINSTITUTIONAL SUBSCRIPTIONS
Send a recommendation to your institution's librarian or information manager to request an extended free trial for all users.
Recommend to your LibrarianIf you are a librarian or information manager, you may request an extended free trial for your academic or corporate institution.
Request an Institutional Trialyour institution might be eligible for free access via our sponsorship scheme. Check our sponsorship page.
If you think you should be able to access this content, please contact us.