Vesicle motion and fusion are altered in chromaffin cells with increased SNARE cluster dynamics.
Traffic. 2009 Feb; 10(2):172-85
This article PDF usually requires a subscription to access but you can receive the PDF, at no cost, through a special arrangement between Springer Science+Business Media and Faculty of 1000 for our Faculty Members who act as reviewers. This PDF is provided to you only for the purposes of reviewing the article while you consider preparing a recommendation of the article for F1000Prime. Other than for this purpose you warrant that you will treat the PDF as confidential. Furthermore you warrant that you will not use the PDF for any other purpose, especially but not limited to forwarding, copying, selling, distributing it whether commercially or non-commercially and that you will not make it available on any website for any other purpose.
I found this paper interesting because of the measurement of the dynamics of SNARE-clusters and the implications for vesicle fusion probability.
Clusters of GFP-linked SNAP-25 in chromaffin cells were visualized under TIRF microscopy and it was found that a deletion of the last C-terminal amino acids led to more dynamic clusters with increased mobility. This mutation corresponds to the cleavage of SNAP-25 by Botulinum Toxin A (the main constituent of BOTOX). Increasing the intracellular calcium concentration stabilized the mutant clusters, which correlates with the observation by several labs that the effect of Botulinum Toxin A can be partly overcome by calcium. Thus, mobility of SNARE-clusters might be important for vesicle fusion, and the paper shows how this mobility can be measured simultaneously with vesicle fusion. A more puzzling result is that the mutant SNAP-25 clusters were also more mobile in the Z-axis (perpendicular to the plasma membrane) and here displayed movements far exceeding the length of the SNAREpin.
Soerensen J: F1000Prime Recommendation of [López I et al., Traffic 2009, 10(2):172-85]. In F1000Prime, 21 Jan 2009; DOI: 10.3410/f.1146942.604040. F1000Prime.com/1146942#eval604040
F1000Prime Recommendations, Dissents and Comments for [López I et al., Traffic 2009, 10(2):172-85]. In F1000Prime, 25 Apr 2014; F1000Prime.com/1146942
Get the most out of F1000Prime - attend a live online demonstration.
Please choose one of the following time zones:
Want to become an
has been added to your "Faculty I'm Following" page in MyF1000
Follow/Unfollow any Faculty via their recommendations, biography pages, or MyF1000
If you've forgotten your password, please enter your email address below and we'll send you instructions on how to reset your password.
The email address should be the one you originally registered with F1000.
You registered with F1000 via Google, so we cannot reset your password.
To sign in, please click here.
If you still need help with your Google account password, please click here.
You registered with F1000 via Facebook, so we cannot reset your password.
To sign in, please click here.
If you still need help with your Facebook account password, please click here.
We have sent an email to , please follow the instructions to reset your password.
If you don't receive this email, please check your spam filters and/or contact email@example.com.