Cellular Death & Stress Responses | Protein Chemistry & Proteomics | Structure: Replication & Repair | Structural Genomics
Insights into DDR: a tale of wet and dry science
Ildefonso Cases, Juan L Rodriguez-Barbancho, Oscar Fernández-Capetillo, Ana M Rojas*
*Corresponding author: Ana M Rojas
IMPPC: Institute for Predictive and Personalized Medicine of Cancer, Badalona, Spain
F1000Posters 2010, 1: 233 (poster) [ENGLISH]
Poster [4.64 MB]
Intelligent Systems for Molecular Biology 2010 meeting, 11 - 13 Jul 2010, W42
The DNA damage response is a phosphorylation-based signal transduction pathway essential for cell viability. Can we expand our knowledge of this pathway using computational biology? We have analyzed the substrates of the ATM and defined how many of them are influenced by replicative stress (the cell cycle). Then, using functional enrichment data from the substrates, we have experimentally addressed the function of specific components at the DNA damage sites, in particular the 53BP1 protein.
We show that TUDOR domain is dispensable for the binding to the DNA and alternative parts of the protein to be involved in the DNA binding. Further comparative analyses with bacterial homologues indicate a role in nucleic acids processing.
No relevant conflicts of interest declared.
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