Virology | Anemias & Hypocellular Marrow Disorders
Inhibition of HIV-1 in sickle cell disease peripheral blood mononuclear cells
Tatyana Ammosova, Sharroya Charles, Altreisha Foster, Sharmin Diaz, Bak Kim, Victor R Gordeuk, Sergei Nekhai*
*Corresponding author: Sergei Nekhai
Center for Sickle Cell Disease, Department of Medicine, Howard University, Washington, USA
F1000Posters 2010, 1: 30 (poster) [ENGLISH]
Poster [247.50 KB]
51st American Society of Hematology Annual Meeting 2009, 5 - 8 Dec 2009, 1509
The hypoxic response is an important component of the body s reaction to impaired tissue oxygenation associated with the anemia and vasoclusive episodes of sickle cell disease (SCD). It has been reported that HIV infection progresses relatively slowly in patients with SCD and we recently showed that HIV-1 transcription and replication is significantly reduced in cells cultured at 3% versus 21% oxygen. Our previous studies indicated that protein phosphatase-1 (PP1) interacts with HIV-1 transcriptional activator, Tat, and thereby participates in the regulation of HIV-1 transcription.
Sickle cell patients are in chronically hypoxic state and we hypothesized that HIV-1 replication in their peripheral blood mononuclear cells (PBMCs) would be slower then in controls.
Our results provide a direct evidence of that HIV-1 replication may be slower in SCD-derived PBMCs. In future, we will analyze PP1 activity and the association of PP1 with regulatory subunits in SCD PBMCs. Understanding how oxygen status influences HIV-1 replication might open new possibilities for treatment of hidden HIV-1 reservoirs that harbor non-replicating HIV-1 virus.
No relevant conflicts of interest declared.
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